Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes.
نویسندگان
چکیده
Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+ uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca2+ levels and mitochondrial Ca2+ uptake in mammalian cells.
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ورودعنوان ژورنال:
- Bio-protocol
دوره 7 5 شماره
صفحات -
تاریخ انتشار 2017